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All Biomarkers/Hormones
20 biomarkers

Hormone Blood Test: Thyroid, Testosterone & More

Hormones orchestrate nearly every process in your body — from metabolism and mood to sleep and sexual health. Our hormonal health panel measures key sex hormones, thyroid markers, and adrenal output so you can detect imbalances early and optimise vitality at any age.

  • Evaluate sex hormones, thyroid & adrenal function together
  • Detect imbalances before symptoms escalate
  • Gender-specific optimal ranges for precision
  • Track hormone optimisation over time

Why this matters

Hormonal imbalances affect energy, weight, mood, and reproductive health. Most standard panels miss critical markers like free testosterone, DHEA-S, and SHBG that reveal the full picture.

Check Your Hormones

Trusted by 2,847+ health optimizers

20 results

Testosterone, Total, Ms

ng/dL

Testosterone is essential for male health, influencing everything from physical strength to mental clarity. Functional medicine approaches focus on addressing root causes of low testosterone including nutrient deficiencies, stress, sleep disorders, and environmental toxins.

Optimal Range

Male: 300-1000 ng/dL

optimal 600-900 ng/dL

EssentialAdvancedMax

Testosterone, Free

pg/mL

Free testosterone is the active form that directly impacts masculine vitality, muscle building, fat burning, and sexual function. It's often a better indicator of testosterone deficiency than total testosterone alone.

Optimal Range

Male: 9-30 pg/mL

optimal 15-25 pg/mL

EssentialAdvancedMax

TSH

m[IU]/L

TSH acts as the thyroid's control center, regulating metabolism, energy production, and numerous body functions. Optimal levels support vibrant health and prevent metabolic dysfunction.

Optimal Range

0.5-2.5 mIU/L

optimal 1.0-2.0 mIU/L

EssentialAdvancedMax

T3 Uptake

%

Thyroid

Optimal Range

24 - 35 %

EssentialAdvancedMax

Free T4 Index (T7)

{Index_val}

Thyroid

Optimal Range

1.4 - 3.8 {Index_val}

EssentialAdvancedMax

Testosterone/CRP Ratio

ng/dL

Hormonal Health

EssentialAdvancedMax

Estradiol

pg/mL

Balanced estradiol levels in men support bone health and cognitive function, but excess estradiol can suppress testosterone and cause unwanted feminizing effects.

Optimal Range

Male: 10-42 pg/mL

optimal 20-30 pg/mL

AdvancedMax

Sex Hormone Binding Globulin

nmol/L

SHBG acts as a hormone regulator, controlling the balance between bound and free hormones. Optimal SHBG levels ensure proper hormone delivery to tissues while preventing excessive hormone activity.

Optimal Range

Male: 10-50 nmol/L

optimal 20-40 nmol/L

AdvancedMax

% Testosterone Bioavailable

%

Hormonal Health

AdvancedMax

Testosterone/ApoB Ratio

{ratio}

Hormonal Health

AdvancedMax

Testosterone/Estradiol (T:E2)

{ratio}

Hormonal Health

AdvancedMax

Cortisol, Total

ug/dL

Cortisol orchestrates the body's stress response and daily energy rhythms. Balanced cortisol levels support healthy stress adaptation while preserving testosterone production and overall vitality.

Optimal Range

Morning (8 AM): 10-20 μg/dL

optimal 12-18 μg/dL

Max

Progesterone

ng/mL

Progesterone in men supports neurological health, sleep quality, and serves as a building block for other hormones. Optimal levels indicate healthy hormone synthesis pathways.

Optimal Range

Male: 0.1-1.0 ng/mL

optimal 0.3-0.8 ng/mL

Max

Dhea Sulfate

ug/dL

DHEA-S serves as a hormone reservoir and stress buffer, supporting overall vitality and healthy aging. Optimal levels promote energy, immune function, and hormone balance.

Optimal Range

Male (30-39): 120-520 μg/dL

(40-49): 95-530 μg/dL

optimal upper 1/3 of range

Max

Igf 1, Lc/Ms

ng/mL

Hormones

Optimal Range

115 - 307 ng/mL

Max

Z Score (Female)

{Z-score}

Hormones

Max

Z Score (Male)

{Z-score}

Hormones

Max

Cortisol:DHEA-S Ratio

{ratio}

Hormonal Health

Max

Free T3:Free T4 Ratio

{ratio}

Hormonal Health

Max

T4 (Thyroxine), Total

ug/dL

Thyroid

Optimal Range

6 - 10 ug/dL

Complete Guide

Understanding Hormonal Health Testing

Hormones are chemical messengers that travel through the bloodstream to regulate virtually every biological process — from how fast your heart beats to how your brain processes emotion. The endocrine system, which produces and coordinates hormone secretion, is not a collection of isolated glands but a deeply interconnected signalling network. Disruption at any point — whether in the hypothalamus, pituitary, thyroid, adrenals, or gonads — cascades through the entire system.

Hormonal imbalances affect an estimated one in five adults, yet the symptoms — fatigue, mood changes, low libido, weight gain, and brain fog — are so non-specific that they are frequently dismissed or misattributed. Comprehensive hormone blood testing cuts through this ambiguity by providing objective measurements of the hormones and binding proteins that govern your vitality at every stage of life.

How Hormones Connects Across the Body

Hormones health does not exist in isolation — it is deeply intertwined with every major system.

Brain & Mental Health

Sex hormones and thyroid hormones directly modulate neurotransmitter systems. Oestrogen promotes serotonin and dopamine receptor sensitivity, explaining the mood fluctuations of perimenopause. Low testosterone in men correlates with increased rates of clinical depression. Thyroid hormone deficiency slows neural conduction, producing cognitive fog and low mood that mimic psychiatric conditions. Cortisol excess from adrenal dysregulation damages the hippocampus and impairs memory consolidation.

Cardiovascular System

Oestrogen exerts protective effects on the vascular endothelium, which partly explains why premenopausal women have lower cardiovascular disease rates than age-matched men. Testosterone maintains healthy red blood cell production and vascular tone. Thyroid hormones regulate heart rate, cardiac output, and cholesterol metabolism — hypothyroidism elevates LDL and triglycerides, while hyperthyroidism increases atrial fibrillation risk. DHEA-S is inversely correlated with cardiovascular mortality in large epidemiological studies.

Bone & Muscle

Oestrogen and testosterone are the primary regulators of bone mineral density. After menopause, falling oestrogen accelerates bone loss at 2–3% per year, dramatically increasing fracture risk within a decade. Testosterone drives muscle protein synthesis in both men and women — declining levels from the mid-30s onward contribute to sarcopenia (age-related muscle loss), reduced strength, and slower metabolism. IGF-1, stimulated by growth hormone, regulates both bone formation and muscle growth.

Metabolic Function

Insulin sensitivity is directly influenced by sex hormones. Oestrogen enhances insulin receptor signalling — its decline at menopause contributes to increased visceral fat accumulation and insulin resistance. DHEA-S improves insulin sensitivity and has anti-obesity effects. Cortisol elevates blood glucose by stimulating hepatic gluconeogenesis; chronically elevated cortisol from HPA axis dysfunction drives metabolic syndrome. Thyroid hormones set the basal metabolic rate — hypothyroidism slows metabolism and promotes weight gain even at normal caloric intake.

Reproductive Health

LH and FSH from the pituitary drive ovarian follicle development and ovulation in women, and testicular testosterone production in men. Elevated FSH signals diminishing ovarian reserve. Prolactin suppresses GnRH, disrupting LH and FSH pulsatility — elevated prolactin from pituitary adenoma is a reversible cause of infertility in both sexes. SHBG tightly controls the bioavailable fraction of testosterone and oestradiol, making it a critical contextual variable when interpreting sex hormone levels.

Supporting Hormones Health

Nutrition

  • Prioritise dietary fat — cholesterol is the precursor to all steroid hormones including testosterone, oestrogen, and cortisol. Very low-fat diets suppress sex hormone production.
  • Zinc (found in oysters, red meat, pumpkin seeds) is essential for testosterone synthesis and thyroid hormone receptor function.
  • Cruciferous vegetables (broccoli, Brussels sprouts) support oestrogen metabolism by promoting the beneficial 2-hydroxyoestrone pathway over the proliferative 16-hydroxyoestrone pathway.
  • Minimise ultra-processed foods and seed oils high in omega-6 fatty acids, which promote inflammatory signalling that disrupts hormonal balance.

Exercise

  • Resistance training acutely and chronically elevates testosterone and growth hormone — 3–4 sessions per week of compound movements (squats, deadlifts, rows) is particularly effective.
  • High-intensity interval training (HIIT) improves insulin sensitivity and reduces visceral fat, both of which support healthy hormonal balance.
  • Excessive endurance training without adequate recovery raises cortisol and suppresses LH, FSH, and sex hormones — a pattern called exercise-induced hypothalamic amenorrhoea in female athletes.

Sleep & Stress

  • Testosterone secretion is predominantly nocturnal — even a single night of poor sleep reduces morning testosterone by 10–15%. Consistent 7–9 hours of sleep is a non-negotiable foundation for hormonal health.
  • Cortisol follows a diurnal rhythm with peak levels on waking. Chronic psychological stress dysregulates this rhythm, suppressing sex hormone production via the hypothalamic-pituitary axis.
  • Mind-body practices such as meditation, yoga, and breathwork reduce cortisol and improve HRV, supporting downstream hormonal balance.

Clinical Note

Hormonal testing should be performed under standardised conditions: fasting, in the morning (when testosterone and cortisol peak), and — for women — at a consistent phase of the menstrual cycle. A single abnormal result should always be confirmed with repeat testing before treatment decisions are made.

Who benefits

Who Should Get Hormones Testing?

health conscious

over 30

athletes

women planning pregnancy

Common Questions

Frequently Asked Questions About Hormonal Health Testing

What does a hormonal health blood test measure?

A hormonal health panel measures the concentration of key hormones in your bloodstream, including sex hormones (testosterone, estradiol, progesterone), adrenal hormones (DHEA-S, cortisol), thyroid hormones (TSH, Free T3, Free T4), and binding proteins like SHBG and LH. Together these markers reveal how well your endocrine system is producing and regulating hormones.

What are common symptoms of a hormonal imbalance?

Common symptoms include unexplained fatigue, weight gain or difficulty losing weight, low libido, mood swings, depression or anxiety, irregular or absent periods in women, hair thinning, brain fog, poor sleep, and reduced muscle mass. Because many of these symptoms overlap with other conditions, blood testing is the most reliable way to confirm a hormonal cause.

Who should get a hormonal health test?

Anyone over 30 experiencing fatigue, mood changes, or metabolic slowdown benefits from a hormone panel. It is especially valuable for women navigating perimenopause or menopause, men experiencing andropause symptoms, athletes monitoring performance recovery, and people investigating fertility issues. Baseline testing in your 30s establishes a personal reference point for future comparison.

How often should you test your hormones?

For most adults, testing once or twice a year is sufficient to track trends. If you are actively managing a hormonal condition, using hormone replacement therapy, or trying to optimise performance, quarterly testing allows more precise adjustments. Hormone levels can fluctuate with time of day, stress, and menstrual cycle phase, so consistent testing conditions improve result reliability.

What is the difference between total testosterone and free testosterone?

Total testosterone measures all testosterone in the blood — both bound to proteins (SHBG and albumin) and the unbound "free" fraction. Free testosterone is the biologically active portion that cells can actually use. A person can have normal total testosterone but low free testosterone if SHBG is elevated, which can cause symptoms of deficiency. Measuring both gives a complete picture.

Can hormonal imbalances affect mental health?

Yes. Oestrogen, progesterone, testosterone, thyroid hormones, and cortisol all directly influence neurotransmitter systems including serotonin, dopamine, and GABA. Low thyroid function is strongly linked to depression and cognitive slowing. Low testosterone in men correlates with increased rates of depression. Cortisol dysregulation underpins many anxiety disorders. Identifying and correcting hormonal root causes can significantly improve mental health outcomes.

Clinical References

  1. [1]Endocrine Society: Testosterone Therapy in Men with Hypogonadism — Clinical Practice Guideline (2018)(2018)
  2. [2]NAMS 2022 Hormone Therapy Position Statement — Menopause Journal(2022)
  3. [3]American Thyroid Association: Thyroid Function Tests(2024)
  4. [4]Society for Endocrinology: Guideline for Female Hypogonadism (2024)(2024)
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