% Testosterone Free (Percent Free Testosterone) Biomarker Testing
It shows what percent of your testosterone is unbound and active, helping explain symptoms when totals mislead, with Quest labs via Vitals Vault.
With Vitals Vault, you have access to a comprehensive range of biomarker tests.
% Testosterone Free (often called “percent free testosterone”) tells you what share of your total testosterone is circulating unbound to proteins. That unbound portion is the most immediately biologically active, because it can enter cells and signal directly.
This marker is especially helpful when your symptoms do not match your total testosterone number. If binding proteins change, your total testosterone can look “fine” while the active fraction is lower (or higher) than expected.
Because only a small fraction of testosterone is normally free—often around 1–3%—small shifts in binding can meaningfully change your percent free result. That is why this calculated value is usually interpreted alongside total testosterone, free testosterone, and binding proteins such as SHBG (sex hormone–binding globulin).
Do I need a % Testosterone Free test?
You may want this test if you have symptoms that can fit with low androgen activity, such as fatigue, low libido, erectile dysfunction, mood changes, or reduced muscle mass, but your total testosterone result does not clearly explain what you are feeling.
It can also be useful if you are in a situation where binding proteins commonly shift. For example, aging and certain thyroid or liver patterns can raise SHBG and lower the free fraction, while insulin resistance and higher body fat can lower SHBG and raise the free fraction.
If you are monitoring hormone therapy or trying to understand why your testosterone-related symptoms change over time, percent free testosterone can add context to a single total testosterone number.
Testing supports clinician-directed care and better-informed decisions, but it cannot diagnose the cause of symptoms on its own. Your best next step is to interpret this result together with your overall hormone panel and health history.
This is a calculated ratio (not a standalone hormone assay) and should be interpreted in context with the underlying testosterone measurements and clinical picture.
Lab testing
Order labs to measure Free Testosterone
from $44.49 at Quest Diagnostics
About 1 week
Schedule online — results typically within a week
Clear next steps
Guidance included, with follow-up care available
HSA / FSA
Eligible for pre-tax health spending accounts
Get this test with Vitals Vault
Vitals Vault makes it straightforward to order labs and understand what they mean without waiting weeks for an appointment. If you are comparing symptoms to your hormone numbers, percent free testosterone can be a practical way to see whether binding effects are changing your active testosterone signal.
After you order, you can complete your blood draw through the Quest network. When results are ready, you can use PocketMD to review your numbers, ask questions in plain language, and decide what to discuss with your clinician or what to recheck.
This approach is especially helpful for trend tracking. Percent free testosterone can move when SHBG changes, when weight or insulin sensitivity changes, or when medications affect binding, so having a consistent way to re-test can clarify whether a change is real or just noise.
- Order online and complete your draw through the Quest network
- PocketMD helps you interpret results and plan smart follow-up testing
- Designed for repeat testing so you can track trends over time
Key benefits of % Testosterone Free testing
- Shows how much of your testosterone is in the unbound, most biologically active form.
- Helps explain symptoms when total testosterone looks normal but binding proteins are shifting.
- Adds context for age-related SHBG increases that can reduce active testosterone signaling.
- Supports monitoring when lifestyle changes (weight, insulin sensitivity) may alter SHBG and the free fraction.
- Improves interpretation of hormone panels by highlighting binding effects rather than production alone.
- Can guide more targeted follow-up testing (such as SHBG, albumin, or repeat morning testosterone).
- Makes it easier to track meaningful changes over time using consistent lab ordering and PocketMD review.
What is % Testosterone Free?
% Testosterone Free is the percentage of your total testosterone that is circulating as free (unbound) testosterone. Testosterone in blood exists in three main “states”: tightly bound to SHBG, loosely bound to albumin, and free. The free portion is the fraction that can enter cells most readily and exert biological effects.
In many people, only a small portion of testosterone is free—often roughly 1–3%. That means your percent free result can change even when your body’s testosterone production has not changed, simply because binding proteins have changed.
This is why percent free testosterone is often used as a reality check on total testosterone. Total testosterone reflects the sum of bound and unbound hormone, while percent free testosterone reflects how much of that total is immediately available to tissues.
Your result is not interpreted in isolation. It is most useful when you also know your total testosterone, your free testosterone value, and factors that influence binding (especially SHBG, and sometimes albumin).
Why binding proteins matter (SHBG and albumin)
SHBG (sex hormone–binding globulin) binds testosterone strongly and reduces the free fraction. Albumin binds more loosely, and testosterone can more easily dissociate from it. When SHBG rises, percent free testosterone often falls even if total testosterone stays the same. When SHBG falls, percent free testosterone often rises, sometimes masking low production if you only look at total testosterone.
When percent free is more informative than total testosterone
Percent free testosterone can be especially informative when your total testosterone is borderline, when symptoms and total testosterone do not match, or when you have known reasons for SHBG to be high or low. It can also help you and your clinician decide whether follow-up testing should focus on production (testes/ovaries and pituitary signaling) or on binding and metabolism.
How % Testosterone Free is calculated
Formula
Free Testosterone / Total Testosterone × 100
This calculation expresses free testosterone as a percentage of total testosterone. Your lab report may list the result as a percent (%), and it depends directly on the accuracy and timing of the underlying free testosterone and total testosterone measurements.
Because this is a derived value, it is most reliable when both inputs were measured from the same blood draw and interpreted with the same reference context. If either input is affected by timing (such as afternoon vs morning draws) or by assay differences, the percentage can shift even if your physiology has not meaningfully changed.
What do my % Testosterone Free results mean?
Low % Testosterone Free
A low percent free testosterone result suggests that a smaller share of your total testosterone is unbound and readily available to tissues. One common reason is higher SHBG, which can increase with aging and can also rise with certain liver or thyroid patterns. If your total testosterone is normal but your percent free is low, your symptoms may reflect reduced active testosterone signaling rather than low production alone. A useful next step is to review SHBG (and sometimes albumin) and confirm that your testosterone testing was done under consistent conditions.
In-range % Testosterone Free
An in-range result suggests that the balance between bound and free testosterone is typical for the reference range used by your lab. This does not automatically mean your testosterone status is “perfect,” because total testosterone, free testosterone (absolute value), symptoms, and SHBG can still tell a different story. If you feel well and your related markers are consistent, an in-range percent free result often supports that binding is not the main issue. If symptoms persist, it can help narrow your focus to other contributors such as sleep, thyroid function, medications, mood, or overall metabolic health.
High % Testosterone Free
A high percent free testosterone result means a larger share of your total testosterone is unbound. This can happen when SHBG is lower than usual, which is often seen with obesity or insulin resistance and can also occur with hypothyroid patterns or certain kidney protein-loss conditions. A higher percentage does not always mean you have “high testosterone,” because the total amount may be normal or even low. If your percent free is high, it is worth checking whether SHBG is low and whether metabolic factors are influencing hormone binding.
Factors that influence % Testosterone Free
SHBG is the biggest driver of changes in percent free testosterone, and it can shift with age, thyroid status, liver health, and some medications (including certain anticonvulsants). Body composition and insulin sensitivity also matter, because lower SHBG is commonly associated with higher body fat and insulin resistance. Timing and consistency of testing can influence results as well, since testosterone has a daily rhythm and can vary day to day. Finally, because this is a calculated marker, differences in how free testosterone and total testosterone are measured can affect the percentage, so trending is most meaningful when you repeat testing in a similar way.
What's included
- Testosterone, Free
Frequently Asked Questions
What is a normal percent free testosterone?
Many labs consider only a small fraction of testosterone to be free, often around 1–3%, but “normal” depends on the lab’s reference range and the method used for the underlying free and total testosterone measurements. The most useful interpretation compares your percent free result with your total testosterone, free testosterone (absolute value), SHBG, symptoms, and testing conditions (such as morning vs afternoon draw). If you are trending results, try to use the same lab and similar timing so changes are easier to trust.
Is % free testosterone the same as free testosterone?
No. Free testosterone is an amount (a concentration) of unbound testosterone in your blood. % Testosterone Free is a percentage that describes how large the free portion is relative to your total testosterone. You can have a normal percentage with a low total amount, or a high percentage with a low total amount, so it helps to look at both the percent and the absolute free testosterone value.
Why can my total testosterone be normal but my % free testosterone be low?
This often happens when SHBG is higher than usual. SHBG binds testosterone tightly, which can keep total testosterone in a normal range while reducing the unbound fraction that can act on tissues. Aging, certain thyroid patterns (including hyperthyroidism), liver-related changes, and some medications can raise SHBG. In that situation, checking SHBG and confirming consistent testing conditions can clarify what is driving the mismatch.
What causes a high percent free testosterone?
A higher percentage commonly reflects lower SHBG, which can occur with obesity and insulin resistance and may also be seen with hypothyroid patterns or certain kidney conditions that affect proteins. A high percentage does not automatically mean your total testosterone is high; it may simply mean less of your testosterone is bound. Looking at total testosterone, SHBG, and metabolic markers can help explain why the percentage is elevated.
Do I need to fast for a % free testosterone test?
Fasting is not always required for testosterone testing, but many people schedule hormone labs in the morning and may combine them with fasting metabolic labs. The bigger issue for interpretability is consistency: try to test at a similar time of day, under similar conditions, and avoid major acute stressors when possible. If your clinician gave you specific instructions based on your broader panel, follow those directions.
When is the best time of day to test testosterone and percent free testosterone?
Testosterone tends to be higher in the morning, especially in younger men, and it can vary day to day. For that reason, many clinicians prefer morning testing for comparison and repeatability. If you are monitoring a trend, the most helpful approach is to repeat your labs at a similar time of day and under similar conditions so the percent free calculation reflects physiology rather than timing differences.
Should I also test SHBG if my % free testosterone is abnormal?
Often, yes. SHBG is a key reason percent free testosterone shifts independent of testosterone production. If your percent free is low, SHBG may be high; if your percent free is high, SHBG may be low. Testing SHBG (and sometimes albumin) can help you and your clinician understand whether the issue is primarily binding, production, or a combination.