PSA Total with Reflex to PSA Free Biomarker Testing
It measures PSA in your blood and, if elevated, adds free PSA to refine risk; order through Vitals Vault and test at Quest locations nationwide.
With Vitals Vault, you have access to a comprehensive range of biomarker tests.
This test starts with a PSA total (prostate-specific antigen) blood level and automatically adds a free PSA measurement if your total PSA crosses the lab’s reflex threshold. That “reflex” step matters because the free-to-total pattern can help clarify whether a borderline or mildly elevated PSA is more consistent with benign prostate enlargement (BPH) or warrants closer follow-up.
PSA is not a cancer diagnosis. It is a signal that needs context, including your age, prostate size, urinary symptoms, medications, and whether you recently had anything that can temporarily raise PSA.
If you are deciding whether to screen, recheck an elevated result, or monitor known BPH, this combined approach can reduce unnecessary repeat blood draws and give you a more actionable next-step conversation with your clinician.
Do I need a PSA Total with Reflex to PSA Free test?
You might consider this test if you are discussing prostate cancer screening and you want a result that is easier to interpret than PSA total alone. Many people feel stuck between “screening saves lives” and “screening causes unnecessary worry.” A reflex-to-free PSA approach is designed for that gray zone, because it adds extra information only when it is most useful.
This test is also commonly used when you already have a PSA result that is higher than expected for you, especially if it is in a borderline range where short-term variation is common. If your PSA is elevated, the free PSA component can help your clinician decide whether to repeat the test, look for reversible causes (like inflammation), or consider additional evaluation.
You may also benefit if you have lower urinary tract symptoms that could be from BPH, such as weak stream, hesitancy, or getting up at night to urinate. PSA can rise with BPH, and trending PSA over time can be more informative than a single number.
Testing supports clinician-directed care and shared decision-making. It can help you choose sensible next steps, but it cannot tell you on its own whether you do or do not have prostate cancer.
This is a laboratory blood test typically performed in a CLIA-certified lab; results should be interpreted with your clinical history and are not a standalone diagnosis of cancer.
Lab testing
Order PSA Total with Reflex to PSA Free and build a clean baseline you can trend over time.
Schedule online, results typically within about a week
Clear reporting and optional clinician context
HSA/FSA eligible where applicable
Get this test with Vitals Vault
Vitals Vault lets you order PSA Total with Reflex to PSA Free directly, so you can move from uncertainty to a clear baseline and a plan for follow-up. Because the free PSA measurement is added only when your total PSA meets the reflex criteria, you avoid ordering multiple tests “just in case.”
After your results post, you can use PocketMD to ask practical questions like whether your result should be repeated, what factors could have influenced it, and what additional labs might add context. That is especially helpful if you are trying to decide between watchful waiting, repeating PSA after avoiding confounders, or discussing imaging and referral.
If you are tracking PSA over time, Vitals Vault makes it easier to keep your results organized so you can focus on trend and rate of change, not just one isolated number.
- Order online and test at Quest locations nationwide
- Reflex design adds free PSA only when it’s clinically relevant
- PocketMD helps you turn results into next-step questions
Key benefits of PSA Total with Reflex to PSA Free testing
- Gives you a PSA total baseline you can trend over time rather than relying on a single snapshot.
- Automatically adds free PSA when total PSA is elevated, which can improve interpretation in borderline ranges.
- Helps distinguish patterns more consistent with BPH versus patterns that may warrant closer evaluation.
- Reduces the need for a second blood draw by bundling the most common follow-up test into a reflex workflow.
- Supports shared decision-making about repeating PSA, timing, and whether to discuss imaging or referral.
- Provides a clearer way to monitor PSA changes after prostatitis treatment or other reversible triggers are addressed.
- Pairs well with PocketMD so you can review confounders, risk factors, and an appropriate retesting interval.
What is PSA Total with Reflex to PSA Free?
PSA (prostate-specific antigen) is a protein made mainly by prostate tissue. A small amount normally enters your bloodstream, and the measured value is called PSA total.
In blood, PSA exists in different forms. Some PSA circulates attached to other proteins (bound PSA), and some circulates on its own (free PSA). “PSA Total with Reflex to PSA Free” means the lab measures total PSA first, and if it is above a preset threshold, the lab automatically measures free PSA on the same sample.
The free PSA result is often used to calculate a free-to-total PSA percentage. In general, a lower percentage of free PSA (more PSA is bound) is associated with a higher likelihood that an elevated PSA is related to prostate cancer rather than benign causes, while a higher percentage of free PSA is more consistent with benign enlargement. This is not absolute, but it can be helpful when total PSA is mildly elevated and you are deciding what to do next.
Why PSA can rise even without cancer
PSA can increase when the prostate is larger (BPH), inflamed (prostatitis), irritated by recent ejaculation, or affected by urinary retention or instrumentation. Because PSA is sensitive but not specific, the best interpretation usually combines your PSA value, your symptoms, your exam history, and your prior PSA trend.
What “reflex” means on your lab order
A reflex test is triggered by a predefined rule. If your total PSA is below the reflex cutoff, you typically will not see a free PSA number reported because it was not run. If your total PSA meets the cutoff, the lab runs free PSA automatically, which helps avoid delays and repeat visits.
What do my PSA Total with Reflex to PSA Free results mean?
Low PSA total
A low PSA total is generally reassuring, especially if you have no concerning symptoms and your result is stable over time. PSA can be very low in younger people and may remain low for years. If you are taking medications that shrink the prostate (such as 5-alpha-reductase inhibitors), PSA can be artificially lowered, so your clinician may interpret your value differently. A low PSA does not completely rule out prostate cancer, but it makes clinically significant disease less likely in many screening contexts.
In-range or expected PSA total for you
An “in-range” PSA depends on the lab reference interval and your personal baseline, including age and prostate size. A stable PSA over time is often more meaningful than a single value, so keeping prior results for comparison helps. If your total PSA does not meet the reflex threshold, free PSA may not be reported, which is normal for this test. If you are screening, your next step is usually deciding when to repeat based on your risk factors and prior trend.
High PSA total (with reflex free PSA when triggered)
A high PSA total means more PSA is reaching the bloodstream than expected, but it does not tell you the cause by itself. When free PSA is reported, the free-to-total pattern can add context: a lower free PSA percentage tends to be more concerning, while a higher percentage is more consistent with benign enlargement, especially when total PSA is only mildly elevated. Your clinician may recommend repeating PSA after avoiding temporary triggers, evaluating for infection or inflammation, or discussing additional assessment such as imaging or specialist referral. Rapid rises over time can also matter, so trend and timing are important.
Factors that influence PSA and free PSA
PSA can rise temporarily after ejaculation, vigorous cycling, prostatitis, urinary retention, or recent procedures involving the prostate or urethra. BPH commonly increases PSA because there is more prostate tissue producing it. Certain medications can lower PSA (notably 5-alpha-reductase inhibitors), and that needs to be accounted for when comparing to reference ranges or prior results. Lab methods and reference intervals can vary, so it helps to compare results from the same lab when you are trending over time.
What’s included
- Psa, Total
Frequently Asked Questions
What does “PSA total with reflex to free PSA” mean?
It means your blood sample is tested for PSA total first. If the total PSA is above a preset cutoff, the lab automatically runs free PSA on the same sample and may report a free-to-total PSA percentage to help interpret a borderline elevation.
What is a normal PSA level?
“Normal” depends on the lab’s reference range, your age, and your baseline. Many clinicians focus on your PSA trend over time and whether the value is unexpected for you rather than relying on one universal cutoff.
What is a good free PSA percentage?
In general, a higher percentage of free PSA is more consistent with benign causes of PSA elevation, while a lower percentage can be more concerning. The exact cut points and how they apply to you depend on your total PSA level, age, and overall risk, so it is best used as part of a bigger decision about follow-up.
Do I need to fast for a PSA test?
Fasting is not usually required for PSA testing. What matters more is avoiding temporary triggers that can raise PSA, such as ejaculation or heavy cycling shortly before the blood draw, and letting your clinician know about recent infections, urinary retention, or procedures.
Can sex or exercise raise PSA?
Yes. Ejaculation can temporarily raise PSA in some people, and activities that put pressure on the perineum (like long bike rides) may also affect PSA for a short period. If your result is borderline high, repeating the test after avoiding these triggers is a common next step.
How often should PSA be repeated?
Repeat intervals vary based on age, baseline PSA, risk factors, and whether you are monitoring an elevated value. If PSA is unexpectedly high, a repeat test after a short interval may be used to confirm the result once temporary influences are addressed.
Does a high PSA mean I have prostate cancer?
No. PSA is a sensitive marker but not specific to cancer, and BPH or inflammation can raise it. A high PSA is a reason to look for context, consider repeating the test, and discuss whether additional evaluation is appropriate.